A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility.
Identifieur interne : 000006 ( Main/Exploration ); précédent : 000005; suivant : 000007A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility.
Auteurs : Mst Hur Madina [Canada] ; Md Saifur Rahman [Canada] ; Xiaoqiang Huang [États-Unis] ; Yang Zhang [États-Unis] ; Huanquan Zheng [Canada] ; Hugo Germain [Canada]Source :
- Biology [ 2079-7737 ] ; 2020.
Abstract
Melampsora larici-populina (Mlp), the causal agent of Populus leaf rust, secretes an array of effectors into the host through the haustorium to gain nutrients and suppress immunity. The precise mechanisms by which these effectors promote virulence remain unclear. To address this question, we developed a transgenic Arabidopsis line expressing a candidate effector, Mlp124357. Constitutive expression of the effector increased plant susceptibility to pathogens. A GxxxG motif present in Mlp124357 is required for its subcellular localization at the vacuolar membrane of the plant cell, as replacement of the glycine residues with alanines led to the delocalization of Mlp124357 to the nucleus and cytoplasm. We used immunoprecipitation and mass spectrometry (MS) to identify Mlp124357 interaction partners. Only one of the putative interaction partners knock-out line caused delocalization of the effector, indicating that Arabidopsis protein disulfide isomerase-11 (AtPDI-11) is required for the effector localization. This interaction was further confirmed by a complementation test, a yeast-two hybrid assay and a molecular modeling experiment. Moreover, localization results and infection assays suggest that AtPDI-11 act as a helper for Mlp124357. In summary, our findings established that one of Mlp effectors resides at the vacuole surface and modulates plant susceptibility.
DOI: 10.3390/biology9090294
PubMed: 32947987
Affiliations:
- Canada, États-Unis
- Michigan, Québec
- Montréal, Trois-Rivières
- Université McGill, Université du Québec à Trois-Rivières
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type="wicri:Area/Main/Exploration">000000</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility.</title><author><name sortKey="Madina, Mst Hur" sort="Madina, Mst Hur" uniqKey="Madina M" first="Mst Hur" last="Madina">Mst Hur Madina</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7</wicri:regionArea><orgName type="university">Université du Québec à Trois-Rivières</orgName><placeName><settlement type="city">Trois-Rivières</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Rahman, Md Saifur" sort="Rahman, Md Saifur" uniqKey="Rahman M" first="Md Saifur" last="Rahman">Md Saifur Rahman</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7</wicri:regionArea><orgName type="university">Université du Québec à Trois-Rivières</orgName><placeName><settlement type="city">Trois-Rivières</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Huang, Xiaoqiang" sort="Huang, Xiaoqiang" uniqKey="Huang X" first="Xiaoqiang" last="Huang">Xiaoqiang Huang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Zhang, Yang" sort="Zhang, Yang" uniqKey="Zhang Y" first="Yang" last="Zhang">Yang Zhang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Zheng, Huanquan" sort="Zheng, Huanquan" uniqKey="Zheng H" first="Huanquan" last="Zheng">Huanquan Zheng</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biology, McGill University, 1205 Dr. Penfield Avenue, Montreal, QC H3A 1B1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, McGill University, 1205 Dr. Penfield Avenue, Montreal, QC H3A 1B1</wicri:regionArea><orgName type="university">Université McGill</orgName><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Germain, Hugo" sort="Germain, Hugo" uniqKey="Germain H" first="Hugo" last="Germain">Hugo Germain</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7</wicri:regionArea><orgName type="university">Université du Québec à Trois-Rivières</orgName><placeName><settlement type="city">Trois-Rivières</settlement><region type="state">Québec</region></placeName></affiliation></author></analytic><series><title level="j">Biology</title><idno type="ISSN">2079-7737</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Melampsora larici-populina (Mlp)</i>, the causal agent of <i>Populus</i> leaf rust, secretes an array of effectors into the host through the haustorium to gain nutrients and suppress immunity. The precise mechanisms by which these effectors promote virulence remain unclear. To address this question, we developed a transgenic <i>Arabidopsis</i> line expressing a candidate effector, Mlp124357. Constitutive expression of the effector increased plant susceptibility to pathogens. A GxxxG motif present in Mlp124357 is required for its subcellular localization at the vacuolar membrane of the plant cell, as replacement of the glycine residues with alanines led to the delocalization of Mlp124357 to the nucleus and cytoplasm. We used immunoprecipitation and mass spectrometry (MS) to identify Mlp124357 interaction partners. Only one of the putative interaction partners knock-out line caused delocalization of the effector, indicating that <i>Arabidopsis</i> protein disulfide isomerase-11 (AtPDI-11) is required for the effector localization. This interaction was further confirmed by a complementation test, a yeast-two hybrid assay and a molecular modeling experiment. Moreover, localization results and infection assays suggest that AtPDI-11 act as a helper for Mlp124357. In summary, our findings established that one of <i>Mlp</i> effectors resides at the vacuole surface and modulates plant susceptibility.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32947987</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2079-7737</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>9</Issue><PubDate><Year>2020</Year><Month>Sep</Month><Day>16</Day></PubDate></JournalIssue><Title>Biology</Title><ISOAbbreviation>Biology (Basel)</ISOAbbreviation></Journal><ArticleTitle>A Poplar Rust Effector Protein Associates with Protein Disulfide Isomerase and Enhances Plant Susceptibility.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E294</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/biology9090294</ELocationID><Abstract><AbstractText><i>Melampsora larici-populina (Mlp)</i>, the causal agent of <i>Populus</i> leaf rust, secretes an array of effectors into the host through the haustorium to gain nutrients and suppress immunity. The precise mechanisms by which these effectors promote virulence remain unclear. To address this question, we developed a transgenic <i>Arabidopsis</i> line expressing a candidate effector, Mlp124357. Constitutive expression of the effector increased plant susceptibility to pathogens. A GxxxG motif present in Mlp124357 is required for its subcellular localization at the vacuolar membrane of the plant cell, as replacement of the glycine residues with alanines led to the delocalization of Mlp124357 to the nucleus and cytoplasm. We used immunoprecipitation and mass spectrometry (MS) to identify Mlp124357 interaction partners. Only one of the putative interaction partners knock-out line caused delocalization of the effector, indicating that <i>Arabidopsis</i> protein disulfide isomerase-11 (AtPDI-11) is required for the effector localization. This interaction was further confirmed by a complementation test, a yeast-two hybrid assay and a molecular modeling experiment. Moreover, localization results and infection assays suggest that AtPDI-11 act as a helper for Mlp124357. In summary, our findings established that one of <i>Mlp</i> effectors resides at the vacuole surface and modulates plant susceptibility.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Madina</LastName><ForeName>Mst Hur</ForeName><Initials>MH</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rahman</LastName><ForeName>Md Saifur</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Xiaoqiang</ForeName><Initials>X</Initials><Identifier Source="ORCID">0000-0002-1005-848X</Identifier><AffiliationInfo><Affiliation>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Huanquan</ForeName><Initials>H</Initials><Identifier Source="ORCID">0000-0003-2986-725X</Identifier><AffiliationInfo><Affiliation>Department of Biology, McGill University, 1205 Dr. Penfield Avenue, Montreal, QC H3A 1B1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Germain</LastName><ForeName>Hugo</ForeName><Initials>H</Initials><Identifier Source="ORCID">0000-0002-7046-6194</Identifier><AffiliationInfo><Affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, 3351 boulevard des Forges, Trois-Rivières, QC G9A 5H7, Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>950-231790</GrantID><Agency>Canada Research Chairs</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Biology (Basel)</MedlineTA><NlmUniqueID>101587988</NlmUniqueID><ISSNLinking>2079-7737</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">GxxxG motif</Keyword><Keyword MajorTopicYN="N">effector</Keyword><Keyword MajorTopicYN="N">fungal rust</Keyword><Keyword MajorTopicYN="N">helper protein</Keyword><Keyword MajorTopicYN="N">plant susceptibility</Keyword><Keyword MajorTopicYN="N">protein disulfide isomerase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>07</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>09</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>19</Day><Hour>1</Hour><Minute>2</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>9</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>20</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32947987</ArticleId><ArticleId IdType="pii">biology9090294</ArticleId><ArticleId IdType="doi">10.3390/biology9090294</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>États-Unis</li></country><region><li>Michigan</li><li>Québec</li></region><settlement><li>Montréal</li><li>Trois-Rivières</li></settlement><orgName><li>Université McGill</li><li>Université du Québec à Trois-Rivières</li></orgName></list><tree><country name="Canada"><region name="Québec"><name sortKey="Madina, Mst Hur" sort="Madina, Mst Hur" uniqKey="Madina M" first="Mst Hur" last="Madina">Mst Hur Madina</name></region><name sortKey="Germain, Hugo" sort="Germain, Hugo" uniqKey="Germain H" first="Hugo" last="Germain">Hugo Germain</name><name sortKey="Rahman, Md Saifur" sort="Rahman, Md Saifur" uniqKey="Rahman M" first="Md Saifur" last="Rahman">Md Saifur Rahman</name><name sortKey="Zheng, Huanquan" sort="Zheng, Huanquan" uniqKey="Zheng H" first="Huanquan" last="Zheng">Huanquan Zheng</name></country><country name="États-Unis"><region name="Michigan"><name sortKey="Huang, Xiaoqiang" sort="Huang, Xiaoqiang" uniqKey="Huang X" first="Xiaoqiang" last="Huang">Xiaoqiang Huang</name></region><name sortKey="Zhang, Yang" sort="Zhang, Yang" uniqKey="Zhang Y" first="Yang" last="Zhang">Yang Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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